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pro ngf  (Alomone Labs)


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    Structured Review

    Alomone Labs pro ngf
    The list of primary and secondary antibodies used in Western blot and fluorescence microscopy techniques.
    Pro Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro ngf/product/Alomone Labs
    Average 90 stars, based on 5 article reviews
    pro ngf - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "The Slow-Releasing and Mitochondria-Targeted Hydrogen Sulfide (H 2 S) Delivery Molecule AP39 Induces Brain Tolerance to Ischemia"

    Article Title: The Slow-Releasing and Mitochondria-Targeted Hydrogen Sulfide (H 2 S) Delivery Molecule AP39 Induces Brain Tolerance to Ischemia

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22157816

    The list of primary and secondary antibodies used in Western blot and fluorescence microscopy techniques.
    Figure Legend Snippet: The list of primary and secondary antibodies used in Western blot and fluorescence microscopy techniques.

    Techniques Used: Western Blot, Fluorescence, Microscopy, Marker



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    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Alomone Labs rabbit polyclonal anti pro ngf antibody
    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and <t>MAB2562</t> (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    The list of primary and secondary antibodies used in Western blot and fluorescence microscopy techniques.

    Journal: International Journal of Molecular Sciences

    Article Title: The Slow-Releasing and Mitochondria-Targeted Hydrogen Sulfide (H 2 S) Delivery Molecule AP39 Induces Brain Tolerance to Ischemia

    doi: 10.3390/ijms22157816

    Figure Lengend Snippet: The list of primary and secondary antibodies used in Western blot and fluorescence microscopy techniques.

    Article Snippet: pro-NGF , Alomone , AGP-031 , 1:300.

    Techniques: Western Blot, Fluorescence, Microscopy, Marker

    Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and MAB2562 (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Research in veterinary science

    Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

    doi: 10.1016/j.rvsc.2022.08.015

    Figure Lengend Snippet: Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and MAB2562 (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

    Techniques: Binding Assay

    Fig. 2. Wes results for recombinant Nerve Growth Factor. A. At 0.01 mg/ml protein load, the primary antibody ab6199 detects NGF at molecular weights 19, 32 and 46 kDa. The 19 kDa peak is interpreted as mNGF. B. Protein load is increased to 0.1 mg/ml and primary antibody MAB2562, specific for proNGF, detects peaks at 38 and 45 kDa.

    Journal: Research in veterinary science

    Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

    doi: 10.1016/j.rvsc.2022.08.015

    Figure Lengend Snippet: Fig. 2. Wes results for recombinant Nerve Growth Factor. A. At 0.01 mg/ml protein load, the primary antibody ab6199 detects NGF at molecular weights 19, 32 and 46 kDa. The 19 kDa peak is interpreted as mNGF. B. Protein load is increased to 0.1 mg/ml and primary antibody MAB2562, specific for proNGF, detects peaks at 38 and 45 kDa.

    Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

    Techniques: Recombinant

    Fig. 4. ProNGF detected in OA chondrocytes by monoclonal antibody MAB2562. A. Wes results: The green curve shows the LPS stimulated (LPS) sample and the blue curve shows the control (C). Peaks detected above 220 kDa are non-migrated proteins and are not considered significant. B. Comparison of Wes data and western blot (far right). Both methods show proNGF at 40 and 45 kDa. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Research in veterinary science

    Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

    doi: 10.1016/j.rvsc.2022.08.015

    Figure Lengend Snippet: Fig. 4. ProNGF detected in OA chondrocytes by monoclonal antibody MAB2562. A. Wes results: The green curve shows the LPS stimulated (LPS) sample and the blue curve shows the control (C). Peaks detected above 220 kDa are non-migrated proteins and are not considered significant. B. Comparison of Wes data and western blot (far right). Both methods show proNGF at 40 and 45 kDa. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

    Techniques: Control, Comparison, Western Blot